SOX2_1

 

 

 

 

A

C

G

T

 

Name

SOX2

1

1331

329

2564

789

 

TF group

HMG

2

5014

18

92

37

 

Polypeptide used

DBD

3

5014

200

43

15

 

Ligand sequence

TAGTAA20NATT

4

0

5014

0

13

 

Batch of SELEX experiment

AC

5

5014

0

0

0

 

Seed sequence

NAACAATNNNATTGTTN

6

5014

15

0

0

 

Hamming distance of sequences to seed

1

7

0

3

0

5014

 

SELEX cycle

4

8

282

11

4132

589

 

Number of repeats

dimeric

9

673

410

2248

1682

 

Comment

possible binding to single stranded DNA

10

215

1515

14

3269

 

Representative matrix

no

11

5014

4

0

3

 

 

 

12

4

5

2321

5014

 

 

 

13

87

3

3

5014

 

 

 

14

0

3

5014

0

 

 

 

15

39

3

49

5014

 

Source:

 

16

127

214

174

5014

 

Jolma et al., Cell 152:327-339 (2013); PubMed 23332764

17

1302

1838

1034

839

 

 

 

 

 

 

 

 

 

 

 

 

SOX2_2

 

 

 

 

A

C

G

T

 

Name

SOX2

1

7055

2944

3078

1412

 

TF group

HMG

2

237

40

67

14489

 

Polypeptide used

DBD

3

109

3

14489

0

 

Ligand sequence

TAGTAA20NATT

4

14489

3

2

4

 

Batch of SELEX experiment

AC

5

14489

65

11

1

 

Seed sequence

NTGAATNNNATTCAN

6

1

4

0

14489

 

Hamming distance of sequences to seed

1

7

1126

2

11454

1907

 

SELEX cycle

4

8

1927

1664

4756

6141

 

Number of repeats

dimeric

9

435

4433

45

9576

 

Comment

possible binding to single stranded DNA

10

14489

8

4

2

 

Representative matrix

no

11

7

10

3146

14489

 

 

 

12

4

5

0

14489

 

 

 

13

0

14489

0

7

 

Source:

 

14

14489

24

26

48

 

Jolma et al., Cell 152:327-339 (2013); PubMed 23332764

15

2465

2393

4052

5579

 

 

 

 

 

 

 

 

 

 

 

 

SOX2_3

 

 

 

 

A

C

G

T

 

Name

SOX2

1

105

62

131

54

 

TF group

HMG

2

352

17

56

17

 

Polypeptide used

DBD

3

7

2

7

352

 

Ligand sequence

TCCCCT20NCCC

4

0

352

0

0

 

Batch of SELEX experiment

AC

5

352

0

0

0

 

Seed sequence

NATCAATNNNATTGATN

6

352

0

0

0

 

Hamming distance of sequences to seed

1

7

0

0

0

352

 

SELEX cycle

4

8

19

0

296

38

 

Number of repeats

dimeric

9

49

55

115

132

 

Comment

possible binding to single stranded DNA

10

34

98

0

220

 

Representative matrix

no

11

352

0

0

0

 

 

 

12

0

0

85

352

 

 

 

13

0

0

0

352

 

 

 

14

0

0

352

0

 

 

 

15

352

0

5

23

 

Source:

 

16

102

20

66

352

 

Jolma et al., Cell 152:327-339 (2013); PubMed 23332764

17

114

20

150

67

 

 

 

 

 

 

 

 

 

 

 

 

SOX2_4

 

 

 

 

A

C

G

T

 

Name

SOX2

1

72

108

146

103

 

TF group

HMG

2

430

20

8

5

 

Polypeptide used

full

3

430

6

0

1

 

Ligand sequence

TGAATA20NTAA

4

0

430

0

0

 

Batch of SELEX experiment

AF

5

430

0

0

1

 

Seed sequence

NAACAATANCATTGTTN

6

430

131

0

2

 

Hamming distance of sequences to seed

1

7

0

0

0

430

 

SELEX cycle

4

8

430

0

90

83

 

Number of repeats

dimeric

9

172

105

87

66

 

Comment

possible binding to single stranded DNA

10

0

430

0

0

 

Representative matrix

no

11

430

0

0

0

 

 

 

12

0

0

0

430

 

 

 

13

0

0

0

430

 

 

 

14

2

0

430

2

 

 

 

15

2

8

3

430

 

Source:

 

16

6

7

9

430

 

Jolma et al., Cell 152:327-339 (2013); PubMed 23332764

17

81

207

45

97

 

 

 

 

 

 

 

 

 

 

 

 

SOX2_5

 

 

 

 

A

C

G

T

 

Name

SOX2

1

21

25

3

35

 

TF group

HMG

2

85

15

2

5

 

Polypeptide used

full

3

2

6

0

85

 

Ligand sequence

TGAATA20NTAA

4

0

85

2

0

 

Batch of SELEX experiment

AF

5

85

0

0

0

 

Seed sequence

NATCAATANCATTGATN

6

85

21

0

0

 

Hamming distance of sequences to seed

1

7

0

0

0

85

 

SELEX cycle

4

8

85

0

1

15

 

Number of repeats

dimeric

9

35

25

14

11

 

Comment

possible binding to single stranded DNA; < 100 counts

10

0

85

0

4

 

Representative matrix

no

11

85

0

0

0

 

 

 

12

0

0

0

85

 

 

 

13

0

0

0

85

 

 

 

14

0

0

85

0

 

 

 

15

85

0

0

1

 

Source:

 

16

1

6

4

85

 

Jolma et al., Cell 152:327-339 (2013); PubMed 23332764

17

36

29

3

17

 

 

 

 

 

 

 

 

 

 

 

 

SOX2_6

 

 

 

 

A

C

G

T

 

Name

SOX2

1

467

99

247

188

 

TF group

HMG

2

8

2

8

1001

 

Polypeptide used

full

3

6

2

1001

2

 

Ligand sequence

TGAATA20NTAA

4

1001

0

0

3

 

Batch of SELEX experiment

AF

5

1001

123

0

2

 

Seed sequence

NTGAATWNCATTCAN

6

0

0

0

1001

 

Hamming distance of sequences to seed

1

7

721

13

191

280

 

SELEX cycle

4

8

361

255

179

206

 

Number of repeats

dimeric

9

6

1001

2

0

 

Comment

possible binding to single stranded DNA

10

1001

2

0

0

 

Representative matrix

no

11

0

1

130

1001

 

 

 

12

0

0

0

1001

 

 

 

13

0

1001

2

3

 

Source:

 

14

1001

16

6

16

 

Jolma et al., Cell 152:327-339 (2013); PubMed 23332764

15

155

227

102

517